Shimakawa et al. (2024): Diatom pyrenoids are encased in a protein shell that enables efficient CO2 fixation
Ginga Shimakawa, Manon Demulder, Serena Flori, Akihiro Kawamoto, Yoshinori Tsuji, Hermanus Nawaly, Atsuko Tanaka, Rei Tohda, Tadayoshi Ota, Hiroaki Matsui, Natsumi Morishima, Ryosuke Okubo, Wojciech Wietrzynski, Lorenz Lamm, Ricardo D. Righetto, Clarisse Uwizeye, Benoit Gallet, Pierre-Henri Jouneau, Christoph Gerle, Genji Kurisu, Giovanni Finazzi, Benjamin D. Engel∙ Yusuke Matsuda IN: Cell, https://doi.org/10.1016/j.cell.2024.09.013
Here, the authors used in vivo photo-crosslinking to identify pyrenoid shell (PyShell) proteins, which we localized to the pyrenoid periphery of model pennate and centric diatoms, Phaeodactylum tricornutum and Thalassiosira pseudonana. In situ cryo-electron tomography revealed that pyrenoids of both diatom species are encased in a lattice-like protein sheath.